For T7-promoter workflows
Prima RNApols® 7 Pro
Prima RNApols® 7 Pro is a T7-promoter compatible RNA polymerase designed to improve RNA synthesis while preserving existing T7-promoter DNA templates. No promoter redesign required.
Contains the following:
- 20,000 U RNA Polymerase (100 µL)
- 2 kb Linearized DNA Template (25 µL)
- 5x Reaction Buffer (100 µL), 4 vials
$329.00
For GMP, bulk orders, or formal quotations, please submit your request.
Performance Data
Translatability of 1.3 kb GFP template measured by fluorescence. The same amount of RNA generated by either polymerase was transfected.
Higher Capping Efficiency & Yield
Ultra-Low dsRNA & Improved Translatability
Capping efficiency determined by LC-MS; dsRNA determined by dot-blot; similar IVT conditions used for each IVT.
Cumulative immunogenicity score: sum of HEK-Lucia RIG-I and THP1-Dual reporter responses. % decrease vs. T7 shown above each bar. Lower dsRNA levels reduce activation of innate immune sensors, consistent with the lower immunogenicity observed.
Yield determined by A260; Integrity determined by fragment analyzer; similar IVT conditions used for each IVT.
Technical Resources
FAQs
What is Prima 7 Pro?
Prima 7 Pro is a T7-promoter compatible RNA polymerase for in vitro transcription (IVT). It supports improved translatability, yield, capping efficiency and ultra-low dsRNA across templates.
Does Prima 7 Pro work with the T7 promoter?
Yes. Prima 7 Pro is compatible with existing T7-promoter DNA templates, allowing teams to evaluate Prima RNApols performance benefits without promoter redesign.
Can I use my preferred capping strategy?
Prima 7 Pro supports co-transcriptional and enzymatic capping strategies and flexible transcription start-site designs. Confirm the suitability of your selected cap strategy and template design during workflow evaluation.
What level of capping efficiency can I expect?
Prima 7 Pro shows higher capping efficiency than T7 across tested AG-capped, AU-capped and self-amplifying RNA (saRNA) conditions. Capping performance varies with the template and reaction conditions. To reach >95% capping efficiency in reactions with 9 mM NTPs, we suggest using 4 mM AG cap concentration. In IVT reactions with 5 mM NTPs, less cap analog is required to reach 95% capping efficiency.
What type of yield can I expect?
Prima 7 Pro delivered RNA yield comparable to or higher than T7 across tested constructs. RNA integrity was template dependent, so both yield and integrity should be evaluated during workflow optimization.
Has Prima 7 Pro been tested with modified RNA?
Yes. Testing has been performed with m1ψ on constructs ranging in size from 1.3 to 9.5 kb. Confirm performance with your selected sequence and nucleotide chemistry.
What improvement in translatability can I expect?
RNA produced with Prima 7 Pro showed 1.6x higher translatability than T7-produced RNA in a reporter assay with equal RNA input. Performance was measured as the area under the protein-expression curve.
Has Prima 7 Pro been evaluated for innate immune activation?
In reporter-cell testing, RNA made with Prima 7 Pro showed 79% lower cumulative immunogenicity scores for modified RNA and 78% lower scores for unmodified RNA than T7-produced RNA.
Can Prima 7 Pro be used for self-amplifying RNA?
Yes, Prima 7 Pro has been evaluated with a 9.5 kb saRNA construct.
How does Primrose recommend purifying RNA made with the Prima 7 Pro kit?
After DNase treatment, we recommend standard lithium chloride (LiCl) precipitation.
Is GMP-grade enzyme supply supported?
Prima 7 Pro kits are for research use only (RUO), supporting R&D and process development. Primrose Bio has established capabilities to supply GMP-grade RNA polymerases as programs advance.
How do I choose between Prima 7 Pro, Prima Cap AG, and Prima Cap AU?
Choose Prima 7 Pro to retain existing T7-promoter DNA templates with AG and AU capping flexibility. Prima Cap AG is designed for AG-capped workflows with template design flexibility, with an overall improvement in translatability, high capping efficiency and even lower dsRNA, even when compared to Prima 7 Pro. Prima Cap AU is designed for AU-capped workflows with template design flexibility, supporting high capping efficiency and ultra-low dsRNA.
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Product purchased through this website is for purchaser’s internal research use only and any other use is prohibited except pursuant to a separate agreement between Primrose Bio and purchaser expressly permitting such other use.