Prima RNApols®: Optimized RNA Polymerases for Consistent, Scalable Manufacturing

Primrose Bio provides comprehensive solutions for low cost and high-quality RNA manufacturing powered by Prima RNApols® — the highest performing RNA polymerases available.

Custom RNApol Evaluation

Tailored RNApol Improvement

RNApols for GMP Manufacturing

Purchase Catalog Prima RNApols

Custom RNApol Evaluation

Tailored RNApol Improvement

RNApols for GMP Manufacturing

Prima RNApols Key Benefits:

By outperforming the industry-standard T7 RNA polymerase, our Prima RNApols improve the cost and quality profiles of pharmaceutical mRNA manufacturing across mRNA formats (linear, self-amplifying, circular) and a range of different DNA template sequences and lengths, as well as a variety of capping chemistries, modified nucleotides and IVT conditions.
Improved Quality Maximize yield of target mRNA and minimize undesirable side-products such as double-stranded RNA (up to 100x reduction).
Efficient Achieve 5-10x higher yield with less DNA template required, significantly reducing production costs.
Customizable Our panel of Prima RNApols and enzyme improvement capabilities allows us to customize solutions that meet our client’s unique mRNA production needs.
Cost Reduction Achieve high capping efficiency and better cap analog utilization, reducing mRNA manufacturing costs and enhancing quality and performance of your mRNA drug substance.

Custom RNApol Evaluation

Primrose Bio has developed a panel of over 20 different RNA polymerases. We screen this panel to identify enzymes that deliver high IVT performance using your materials and to your desired Key Performance Indicators (KPIs). These include yield, dsRNA, integrity, capping efficiency and more.

  • Leverage the power of the panel
  • Identify an RNApol suited for your product and process
Automated liquid handler pipetting into a 96-well plate

Evaluation Program Details

01
Screening (Weeks 1-3):

We identify 20+ enzymes tailored to your target mRNA substance and KPIs, such as yield, dsRNA content, integrity, and capping efficiency.

02
Process Optimization (Weeks 4-7):

We fine-tune IVT conditions and transfer optimized protocols for seamless integration into your applications.

03
Comprehensive Analytics (Weeks 6-8):

We perform best-in-class enzyme and mRNA analytics to ensure precision and quality.

04
Selection (Week 9-10):

Best-performing RNA polymerases are then shipped to you for further analysis, along with the resulting mRNA products.  

TIMELINE

6-10 WEEKS

COST

STARTING AT $20,000

Tailored RNApol Improvement

Enzymes are flexible biocatalysts that can be evolved to perform with the substrates and conditions they are required to operate in. Primrose Bio has developed an industry leading, ultra-high-throughput enzyme evolution workflow to tailor RNA polymerases for mRNA manufacturing. We improve RNApols from our panel efficiently and rapidly to meet your IVT KPIs. We support this effort with high quality enzyme and mRNA analytics from our best-in-class analytical sciences team.

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RNApols customized to your specification
High-quality mRNA analytics

RNApols for GMP Manufacturing

Primrose is fully equipped to supply custom Prima RNApols for GMP manufacturing to customers, providing industry-standard solutions for the most demanding applications.

Scientist operating a bioprocess system in a manufacturing lab

60+

60+ PARTNERSHIPS IN INNOVATIVE PROTEIN AND NUCLEIC ACID-BASED THERAPEUTICS.

DOWNLOAD PRIMA RNAPOLS OVERVIEW IN PDF

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